Table 1.
Name, sequence, applied amount and sources of oligonucleotide probes for Fluorescence in situ hybridization (FISH) analysis.
| Probe Name | Sequence and Fluorochrome Label | The Amount of Each Slide (ng/Slide) | Sequences Used to Develop Probes (GenBank Accession Number) |
|---|---|---|---|
| Oligo-HvT01 | Tamra-5′AAACT CGCAT TTTTG GCCTA TTCTG GCTAG TTCTG CATGC TATTG CTCAC TGATT TTGG3′ | 11.6 | Barley relic DNA HVT01, tandemly repeated sequence (X16095.1) |
| Oligo-pTa71-1 | 6-FAM-5′GACGC GCGCC ATGGA AAACT3′ | 10.8 | Wheat ribosomal DNA (rDNA) 25S-18S intergenic region EcoRI-BamHI fragment (X07841.1) |
| Oligo-s120.1 | 6-FAM-5′TATCG AGTGC GAGTG AGATA TGCAT GTGTA TGTGT GACCC AGGTG GATGG AGAGT TTGA3′ | 21.8 | Triticum aestivum clone pTa-s120 FISH-positive repetitive sequence (KC290913.1) |
| Oligo-s120.2 | 6-FAM-5′GGAGA GGGAT GAACA AGGTT TTTGT GTCGG ATGCA TGCGA CAGAA TTGAA GATTG TCGG3′ | 21.4 | |
| Oligo-s120.3 | 6-FAM-5′GAGAG AGAGA GAGAG AGAGA GAGAG AGAGA GAGAG AGAGA GAGAG AGAGA GA3′ | 19.0 | |
| Oligo-275.1 | 6-FAM-5′TGCTA CTGCT GCTAC TGCTG CTACT CCTGC TACTG CTACT ACTGC TACTC CTGCT ACTC3′ | 24.6 | Triticum aestivum clone pTa-275 FISH-positive repetitive sequence (KC290911.1) |
| Oligo-275.2 | 6-FAM-5′CTACT ACTAC TACTA CTACT ACTAC TACTA CTACT ACTAC TACTA CTACT ACTAC TACT3′ | 22.6 | |
| Oligo-k566 | 6-FAM-5′ATCCT ACCGA GTGGA GAGCG ACCCT CCCAC TCGGG GGCTT AGCTG CAGTC CAGTA CTCG3′ | 23.3 | Triticum aestivum clone pTa-k566 FISH-positive repetitive sequence (KC290904.1) |
| Oligo-713 | 6-FAM-5′GTCGC GGTAG CGACG ACGGA CGCCG AGACG AGCAC GTGAC ACCAT TCCCA CCCTG TCTA3′ | 23.0 | Triticum aestivum clone pTa-713 FISH-positive repetitive sequence (KC290900.1) |