Table 1.
Bacterial strains.
E. coli Strain | Relevant Genotype | Notes |
---|---|---|
MC4100 | ∆(argF-lac)U169 | K-12; gift from J. Scott (Emory University, Atlanta, GA, USA) |
WS3294A | csuBA1A2CD | ETEC CS14+ ST+, human clinical isolate; gift from S. Savarino (Naval Medical Research Center, Silver Spring, MD, USA) |
GPM1124 | attBHK022::pCS14Lac1 | csuBp −228 to +117 1, wild-type * |
GPM1132 | attBHK022::pCS14Lac2 | csuBp −228 to +461, wild-type * |
GPM1140a | attBHK022::pCS14Lac4 | csuBp −228 to +461, mutagenized csuB1o * |
GPM1291a | attBHK022::pCS14Lac3 | csuBp −228 to +461, mutagenized cusB2o * |
GPM1289a | attBHK022::pCS14Lac7 | csuBp −228 to +461, mutagenized csuB3o * |
GPM1321a | attBHK022::pCS14Lac8 | csuBp −228 to +461, mutagenized csuB1o, csuB2o * |
GPM1322a | attBHK022::pCS14Lac9 | csuBp −228 to +461, mutagenized csuB1o, csuB3o * |
GPM1323a | attBHK022::pCS14Lac10 | csuBp −228 to +461, mutagenized csuB2o, csuB3o * |
GPM1324a | attBHK022::pCS14Lac11 | csuBp −228 to +461, mutagenized csuB1o, csuB2o, csuB3o * |
GPM1287a | attBHK022::pCS14Lac6 | csuBp −114 to +461, deleted csuB3o * |
GPM1286b | attBHK022::pCS14Lac5 | csuBp −73 to +461, deleted csuB2o, csuB3o * |
1 Relative to Rns-dependent transcription state site. * From this study.