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. 2016 Dec 14;7(12):124. doi: 10.3390/genes7120124

Figure 2.

Figure 2

Quantitative real-time PCR (qRT-PCR) analysis confirms differential miR expression patterns in SNB from patients with poor prognosis. Comparative miR expression analysis showing up-regulation of miR-574-5p, miR-1246, miR-1182, miR-514b-5p and miR-584-5p (A) and downregulation of miR-214-3p, miR-199-3p, miR-193a-3p, let-7e-5p, miR-365-3p, miR-30c-5p, miR-23a-3p, miR-193b-3p and miR-4299 (B) in the PP compared to the PN samples. Twenty-eight SNB samples from patients relapsing (PP, n = 16) or not (PN, n = 12) within five years of follow-up were tested. The relative quantification was determined using 2−∆Ct, and U6 snRNA was used as the internal reference. Statistical analysis was performed by applying the non-parametric Mann-Whitney U-test. * p < 0.05, ** p < 0.01.