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. 2016 Dec 14;7(12):124. doi: 10.3390/genes7120124

Figure 9.

Figure 9

miR-23a-3p downmodulation is associated with upregulated CD30 expression in myeloid-derived suppressor cells (MDSCs). (A) qRT-PCR analysis showing that peripheral blood mononuclear cells (PBMCs) stimulated with melanoma-conditioned medium upregulate expression of TNFRSF8 and downregulate miR-23a-3p. The 2−∆∆Ct quantification was determined using non stimulated PBMCs as the calibrator, while U6 snRNA was used as the internal reference. * p < 0.05 by unpaired t-test; (B) Gating strategy for MDSC identification: MDSCs were identified inside live cells as CD14+CD3− with a low/negative expression of HLA-DR; (C) Contour plots show the expression of CD30 in MDSCs. Percentage of positive cells, based on isotype IgG control, is indicated for non-activated and for activated lymphocytes.