Ability of whole Borrelia lipid extracts and individual Borrelia lipids to form vesicles with ordered-state bilayers as assayed by fluorescence anisotropy. Samples contained 0.1 mol % DPH in vesicles containing 50 μM lipid. (A) Whole lipid extracts from B. burgdorferi strain B31 (diamonds), B. burgdorferi strain B313 (squares), and B. hermsii (triangles). DOPC (circles) is shown for comparison. (B) Individual lipids ACGal (diamonds), MGalD (squares), B. burgdorferi PC (triangles), and DOPC (circles). The average and standard deviation from three samples are shown.