Figure 3.

Dwell time analysis for PolC-PAmCherry. (a) Scheme for time-lapse imaging. Every imaging frame is captured with a 50-ms integration time (solid rectangles), and a time delay (dashed arrows) is introduced between each pair of consecutive frames. The time-lapse period (τ_TL) is the sum of the integration time and the time delay. (b) Dwell time distributions for PolC-PAmCherry in untreated cells. For clarity, the distributions are shown as stem plots, and the color corresponds to τ_TL as described in (a). (Solid lines) Fits to the exponential decay in Eq. 3. (Inset) Linear fit (black line) of (k_{diss_app} × τ_TL) versus τ_TL, from which the dissociation rate constant k_diss, the photobleaching rate constant k_bleaching, and the dwell time constant τ_dwell are obtained according to Eq. 4. Errors bars are from four rounds of bootstrapping. (c) Dwell time distributions and analysis for PolC-PAmCherry in HPUra-treated cells. To see this figure in color, go online.