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. 2016 Dec 20;111(12):2570–2586. doi: 10.1016/j.bpj.2016.11.007

Figure 4.

Figure 4

ITC results for SF11–255 (white) or phospho-SF11-255 (gray) titrated with B3P3 RNA. (A) Schematic diagram of the ITC experiment. (B and C) Representative isotherms fit with identical-site binding models. Titrations were of (B) 60 μM B3P3SUBOPT RNA into 5 μM SF11-255, c = 125; and (C) 60 μM B3P3SUBOPT RNA into 5 μM phospho-SF11–255, c = 64. Nonidentical-site models were discarded based on 1) little reduction in χ2 for nonidentical sites compared to identical sites; 2) lower data/parameter ratio; and 3) fitting errors greater than the resulting values. (D) Bar graph of apparent affinities (KA) of the interactions, colored as in (A). The apparent equilibrium dissociation constants (KD = KA−1) and the standard deviations between at least two replicates are given.