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. 2016 Dec 20;111(12):2570–2586. doi: 10.1016/j.bpj.2016.11.007

Figure 7.

Figure 7

ITC results for SF1mut (striped) or SF1pa (stippled) titrated with B3P3 RNA. (A) Schematic diagram of the ITC experiment. (B and C) Representative isotherms fit with identical-site binding models for (B) 50 μM B3P3SUBOPT RNA into 5 μM SF1pa, c = 44, and (C) 50 μM B3P3SUBOPT RNA into 5 μM SF1mut, c = 27. (D) Bar graph of apparent affinities (KA) of the interactions, colored as in (A). The KA of unmodified SF11–255 (white) is plotted for comparison. The inset shows apparent equilibrium dissociation constants (KD = KA−1) and standard deviations of at least two replicates.