Figure 6.

Activation of the noncanonical pathway of NF‐κB and TGF‐β production in Ido1 ^−/− pDCs transfected with ITIM‐mutants. (A) Expression of p52 and RelB in nuclear and cytoplasmic extracts from Ido1 ^−/− pDCs transfected with the WT and ITIM‐mutated forms of Ido1. The control was represented by Ido1 ^−/− pDCs transfected with a control mRNA. Lamin B1 and β‐tubulin were used as loading control of nuclear and cytoplasmic extracts respectively. One representative experiment is shown. (B) TGF‐β production in the supernatants of Ido1 ^−/− pDCs transfected with the WT and ITIM‐mutated forms of Ido1. WT and Y115E‐transfected Ido1 ^−/− pDCs were gene silenced by mixed siRNAs targeting Ptnp6 and Ptnp11. Results are representative of three experiments (mean ± S.D.). *P < 0.05, **P < 0.01 (experimental versus control sample; Student’s t‐test).