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. 2016 Sep 30;21(1):165–176. doi: 10.1111/jcmm.12954

Figure 7.

Figure 7

Skin test reactivity of mice immunized with pDCs transfected with ITIM‐mutated Ido1. (A) Splenic HY‐pulsed cDCs in combination with a minority fraction (5%) of Ido1 −/− pDCs were transferred into syngeneic C57BL/6 recipient mice to be assayed for skin reactivity to the eliciting peptide. Before the antigen pulsing, pDCs were transfected with the WT or ITIM‐mutated forms of Ido1. The control was represented by Ido1 −/− pDCs transfected with a control mRNA. (B) ITIM1‐(Y115E)‐transfected Ido1 −/− pDCs were gene silenced by mixed siRNAs targeting Ptnp6 and Ptnp11 and used in combination with cDCs for skin reactivity. The control was represented by negative control (NC) siRNA‐transfected Ido1 −/− pDCs. Analysis of skin reactivity of recipient mice (n = 6 per group) to the eliciting peptide is presented as change in footpad weight. Results are representative of three experiments (mean ± S.D.). *P < 0.05, **P < 0.01 (experimental versus control footpads; two‐tailed paired t‐test).