Table 1.

Primer sequences used in this study

Primers	Sense and antisense oligonucleotides
WT	S 5′‐GGCCAAACTAGTGGTCAGTGGAGTAGACAGCA‐3′
	AS 5′‐CTCTTCTGAGTTGGCCTTAGTGCGGCCGCCTCCACT‐3′
Y115E	S 5′‐TATTGCTGTTCCCGAATGCGAGCTCTCAGAG‐3′
	AS 5′‐CTCTGAGAGCTCGCATTCGGGAACAGCAATA‐3′
Y253E	S 5′‐AAGGTCTGCTGGAAGAGGGGGTCTGG‐3′
	AS 5′‐CCAGACCCCCTCTTCCAGCAGACCTT‐3′

Mutated nucleotides are indicated in italics.