Figure 4.

The effects of netrin‐1 were dependent on the expression of CD151. HUVECs were treated with si‐NC or si‐CD151 and cell proliferation, migration and tube formation in response to netrin‐1 treatment (50 ng/ml) were assessed by (A) CCK8 assay, (B) Transwell assay and (C) tube formation assay. (D) Western blot analysis of VEGF and FAK signalling proteins in HUVECs treated with si‐NC or si‐CD151. All experiments were performed in triplicate. The mean ± S.E.M. was calculated from independent experiments. **P < 0.01; ‘NS’ indicates no significant changes observed. FAK, focal adhesion kinase; VEGF, vascular endothelial growth factor.