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. 2016 Sep 7;21(1):35–45. doi: 10.1111/jcmm.12936

Figure 3.

Figure 3

In vitro scratch assay of HDF and supernatants from IAT and HAT. Wound healing of HDF monolayers was evaluated by an in vitro scratch assay. Fibroblasts were incubated with supernatants of samples from IAT and HAT containing varying concentrations of recombinant human D‐DT and neutralizing MIF antibody. Medium containing 10% FCS served as a positive control and medium containing 0.5% FCS served as a negative control. Experiments were performed in the absence and the presence of the proliferation inhibitor Mitomycin C. (A) Supernatants from IAT showed no significant increase in proliferation and migration of fibroblasts when stimulated with D‐DT alone. (B) When D‐DT and anti‐MIF were added simultaneously, fibroblast proliferation and migration were increased significantly. (C and D) Fibroblast migration did not differ when Mitomycin C was added. All measurements were normalized to the negative control, which was set as 1. Data are presented in relative migration distance of fibroblasts ± S.E.M. n = 8–12 from 3 independent experiments, one‐way anova with Tukey's multiple comparisons test. Statistically significant differences are indicated by asterisks (**P < 0.01, ***P < 0.001).