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. 2016 Sep 6;21(1):154–164. doi: 10.1111/jcmm.12953

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MXRA5 targeting has no effect on cell viability or proliferation. MXRA5 was successfully knocked down by means of a specific siRNA. (A) MXRA5 protein expression was assessed by Western blot (B) and RT‐qPCR (*P < 0.001 versus control). (C) No morphological changes were observed in MXRA5 knocked down cells. Contrast phase microscopy. (D) No changes in cell death or proliferation were observed in MXRA5‐silenced cells. DNA was stained with propidium iodide in permeabilized cells and DNA content quantified by flow cytometry.