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. 2016 Aug 25;21(1):121–129. doi: 10.1111/jcmm.12945

Figure 3.

Figure 3

Asthenospermic and oligoasthenospermic men have increased numbers of sperm positive for lipid peroxidation which may modulate sperm motility loss via increased mitochondrial ROS and decreased high mitochondrial membrane potential. Lipid peroxidation (BODIPY C11) of sperm, as determined using BODIPY (581/591) C11 and PI probes, was analysed using CellQuest™ software. The percentage of sperm with green BODIPY (581/591) C11 staining was determined by flow cytometric analysis in human sperm from normospermic (N), asthenospermic (A), and oligoasthenospermic (OA) subjects. (A) Histogram shows significant increase in A (**P < 0.01) compared with N, and OA (***P < 0.001) compared with N or A. (B, C and E) Reveal significantly negative correlations of sperm lipid peroxidation with (B) total motility (P < 0.0001), (C) progressive motility (P < 0.0001), and (E) high membrane potential of sperm (P = 0.0169); whereas there was a positive correlation with (D) sperm mitochondrial ROS (MitoSOX Red) (P = 0.0036) in normospermic (N), asthenospermic (A) and oligoasthenospermic (OA) subjects.