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. 2016 Sep 13;21(1):130–141. doi: 10.1111/jcmm.12947

Figure 5.

Figure 5

T‐CM inhibits fibronectin expression in L929 cells induced by PA‐stimulated myotubes in an IL‐1Ra‐dependent manner. (A) L929 cells, cultured under various conditions as indicated, were harvested and the expression of fibronectin was quantified by RT‐PCR. The co‐culturing of L929 cells with myotubes was performed in a 24‐well transwell plate. The L929 cells were placed in the lower chamber and the myotubes were seeded in the upper chamber. rIL‐1β (100 ng/ml), experimental T‐CM, and rIL‐1Ra (100 ng/ml) were added to the bottom chamber, which contained the L929 cells. The data are presented as the mean ± SEM (*P < 0.05). (B) The protein level of fibronectin was determined by western blot in the same experimental group. The pixel densities of the fibronectin bands were divided by those of the corresponding β‐actin bands for normalization. The data are presented as the mean ± SEM (*P < 0.05).