Figure 7.

Effects of TRP channel antagonists, capsazepine, HC‐030031, M8‐B and BCTC on the activation of high threshold afferents induced by H₂O₂ and by corresponding agonists. (A) Average data of the effect of capsazepine (10 μM) on H₂O₂ (300 μM)‐induced activation of high threshold afferents (n = 8, N = 5). (B) Average data of the effects of capsazepine (10 μM) on capsaicin (0.5 μM)‐induced activation of high threshold afferents (n = 10, N = 5). (C) Average data of the effect of HC‐030031 (10 μM) on AITC (30 μM)‐induced activation of high threshold afferents (n = 11, N = 5). (D) Average data of the effect of M8‐B (3 μM) on H₂O₂ (1 mM)‐induced activation of high threshold afferents (n = 7, N = 5). (E) Average data of the effect of M8‐B (3 μM) on icilin (10 μM)‐induced activation of high threshold afferents (n = 6, N = 5). (F) Average data of the effect of BCTC (10 μM) on H₂O₂ (1 mM)‐induced activation of high threshold afferents (n = 8, N = 5). *P < 0.05, significant effect of channel antagonist. Note that repeated application of H₂O₂ (300 μM) on the same capsaicin‐sensitive high threshold afferents without antagonists produced a non‐significant decay in its responses (see Figure 6B).