Figure 4.

Ageing regulates the expression of miR‐203 targets in mouse aorta. mRNA and protein expression of the validated miR‐203 targets were analysed to determine if ageing regulated their expression in the thoracic aorta. (A) mRNA expression levels of Src, Cav‐1 and Pxn compared in the thoracic aorta from young and aged mice (n = 10–11 mice). (B) XY scatter plots generated to compare the levels of miR‐203 with the mRNA levels of its targets in the aorta from each individual mouse. (C) Typical western blots depicting Src, Cav‐1 and paxillin protein expression in young and aged aorta are shown on the left, with the subsequent quantification comparing their normalized expressions on the right (n = 8 mice). *P < 0.05 (two‐tailed Student's t‐test).