Figure 6.

Overexpression of miR‐203 in young aorta reproduces the ageing phenotype of increased aortic stiffness. (A) Schematic of the protocol for transfection of aortic tissue with miR‐203 mimic or control mimic. (B) miR‐203 expression levels in tissue transfected with miR‐203 mimic or control (n = 4 experiments). (C) Agonist‐induced stiffness increase in young (white, n = 10) versus aged (black, n = 6) aorta and young aorta transfected with scrambled miR mimic control (light grey, n = 10) versus miR‐203 mimic (pattern grey, n = 9). *P < 0.05 from young aorta or # scrambled mimic control (two‐tailed Student's t‐test).