Figure 4.
Propranolol does not affect the expression of key inflammatory cytokines. (A) mRNA levels of cytokines in B16F10 tumours. Propranolol treatment did not significantly change mRNA expression of IL‐1β, IL‐4, IL‐6, IL‐10, IL‐12, IFN‐γ and TNF‐α in tumour samples (n = 10 per group). (B) mRNA levels of IL‐1β, IL‐6 and IFN‐γ in the spleen of melanoma‐bearing mice. Again, propranolol did not significantly change mRNA expression of cytokines (n = 10 per group). Cytokine and chemokine protein profiling in the sera (C) and tumour tissues (D). The samples derived from control and propranolol‐treated melanoma‐bearing mice were pooled (5 mice per group) and analysed by a dot blot immunoassay. Compared to control, variations of protein expression in the group treated with propranolol (20 mg·kg−1·day−1) were small with all proteins showing a fold change of less than a factor of two.