Figure 1.
Cryo‐EM reconstruction of the MamK filament at 6.5 Å. (A) Representative electron micrograph of frozen‐hydrated MamK. The scale bar is in black at the bottom. (B) Representative class average obtained from a 250‐segment subset of the dataset. The 8‐subunit repeat is indicated. (C) Fourier Shell Correlation curve for the 3D reconstruction. The gold‐standard resolution definition of 0.143% is indicated with a blue dotted line. (D) Final 3D reconstruction of the MamK filament, shown at a contour level of 5.0. The complete map is shown in grey on the left, and the two strands are colored in dark blue and khaki, respectively, on the right.