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. 2017 Jan;360(1):14–22. doi: 10.1124/jpet.116.236620

Fig. 4.

Fig. 4.

The role of NOX5-S in SODD expression. (A) A typical image of three Western blot analyses and (B) summarized data showed that overexpression of NOX5-S significantly increased SODD expression in FLO EA cells, indicating that NOX5-S may contribute to the SODD expression (N = 3). (C) Overexpression of NOX5-S by transfection of FLO cells with NOX5-S plasmid remarkably increased the luciferase activity of SODD promoter in FLO cells (N = 3), indicating that NOX5-S–derived ROS may activate SODD promoter. NOX5-S overexpression-induced increase in SODD promoter activity was significantly decreased by the knockdown of NF-κB1 p50 with its siRNA, suggesting that NOX5-S–dependent activation of SODD promoter may be mediated by NF-κB1 p50. (D) In FLO cells, overexpression of NOX5-S significantly decreased caspase 9 activity (N = 6), indicating that NOX5-S–derived ROS may inhibit cell apoptosis. (E) In FLO cells, overexpression of NOX5-S significantly decreased cell apoptosis (N = 3), indicating that NOX5-S–derived ROS may inhibit cell apoptosis. Analysis of variance, ***P < 0.0001, compared with control siRNA + pCMV group; **P < 0.01, compared with control siRNA + NOX5p group; t test, ▴P < 0.05; *P < 0.0001.