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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Nat Chem Biol. 2016 Nov 14;13(1):69–74. doi: 10.1038/nchembio.2232

Figure 3. CYP117 is an ent-kaurene oxidase (KO).

Figure 3

CYP117 converts ent-kaurene (1) into ent-kaurenoic acid (4), as shown here by GC-MS chromatograms with comparison to an authentic standard (std.) of 4 (the relevant mass spectra can be found in Supplementary Fig. 4). Additionally, individual incubations with ent-kaurenol (2) and ent-kaurenal (3) result in efficient conversion to 4, demonstrating the sequential oxidation of C-19 to the alcohol (2), aldehyde (3), and acid (4) by CYP117.