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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Nat Chem Biol. 2016 Nov 14;13(1):69–74. doi: 10.1038/nchembio.2232

Figure 4. CYP114 is an ent-kaurenoic acid oxidase (KAO) and requires FdGA for full functionality.

Figure 4

a) CYP114 converts ent-kaurenoic acid (4) into ent-7α-hydroxykaurenoic acid (5) and ultimately GA12-aldehyde (6) as the major product, as shown here by GC-MS chromatograms with comparison to an authentic standard (std.) of 5 and 6 (the relevant mass spectra can be found in Supplementary Fig. 6), but production of 6 is only observed when FdGA is coexpressed. b) Incubation with 5 does not result in detectable conversion to 6.