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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Nat Chem Biol. 2016 Nov 14;13(1):69–74. doi: 10.1038/nchembio.2232

Figure 6. CYP112 is a GA 20-oxidase.

Figure 6

CYP112 converts GA12 (7) into GA9 (10) via GA15 (8) and GA24 (9) as shown here by GC-MS chromatograms with comparison to authentic standards (stds.) of 8, 9, and 10 (the relevant mass spectra can be found in Supplementary Fig. 12). 8 and 9 also both act as substrates for CYP112, showing sequential oxidation of C-20 to the alcohol (8) and aldehyde (9) before being lost to form 10. The selected ions for each chromatogram are as follows: +7 m/z = 226, 300; +8 m/z = 270, 284; +9 m/z = 226. *Contaminating substance found in 8 standard used for incubations.