Table IV.
Primers used to generate truncation constructs for Lec8 expression
| UGT1 variant | Primer sequence |
|---|---|
| Full length | 5′-ATCGAATTCGCCCATCGAACCAACACT-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| N1 | 5′-ACTAGAATTCAGAGATCGAAGCGAGAGA-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| N2 | 5′-ACTAGAATTCGGGAAGACGCGCGGTATGGA-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| N3 | 5′-ACTAGAATTCTCCTCTACCTCTGTCAGCT-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| N4 | 5′-ACTAGAATTCGTGGGCTATGCTACTGCGA-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| N5 | 5′-ACTAGAATTCCACTATTCACGGATATCTA-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| N6 | 5′-ACTAGAATTCACTGCATCTCCTCCTC-3′ |
| 5′-TTAACCATGCTTTCTATC-3′ | |
| C1 | 5′-ATCGAATTCGCCCATCGAACCAACACT-3′ |
| 5′-ACACTCGAGACTGGCAGGTGGAGCGGGGCT-3′ |