Table 1. Published selectivity of PKA/EPAC activators and inhibitors.

from cell culture assays. The volume:conc (concentration) values listed in column 1 were concentration and volume used for intrathecal injections in the present in-vivo study.

Drug (volume:conc)	EPAC Ka	PKA Ka	PKA Ki	EPAC Ki
6-Bnz-cAMPa (10μL:100μM)	NSa	2.7μM	-	-
8-pCPT-2′-O-Me-cAMPa (10μL:100μM)	1.8μM	190μM	-	-
Rp-8-Br-cAMPa (10μL:1mM)	-	-	8.5μM	NSi
ESI-05b (10μL:2M)	-	-	NSi	0.43μM

^Kaconcentration for half of maximum cAMP induced response

^Kiconcentration for inhibition of half maximum cAMP induced response

^NSanon-significant activating effect; ≥100 fold Ka difference

^NSinon-significant inhibitory effect; ≥100 fold Ki difference

^aPoppe et al. 2008;

^bTsalkova et al. 2012