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. Author manuscript; available in PMC: 2016 Dec 28.
Published in final edited form as: Cell Stem Cells Regen Med. 2016 May 30;2(2):10.16966/2472-6990.111. doi: 10.16966/2472-6990.111

Figure 2. Porcupine inhibitor treatment inhibits WNT pathway activity in the infarcted heart.

Figure 2

(A) Schematic summarizing animal study timelines. Mice were treated with daily intravenous injection of 5 mg/kg drug or vehicle following MI and continued through day 6. For cardiac recovery studies, mice underwent echocardiography at days 7 and 30. For histology, a separate cohort of mice was sacrificed on days 3, 7 and 15. (B) β-catenin immunostaining of peri- infarct region of ventricles at day 7 showed reduction in β-catenin levels with GNF-6231 treatment. (B′) High magnification image of vehicle-treated tissue showed nuclear localization of β-catenin signifying WNT pathway activation. (C) β-galactosidase immunostaining in ventricle sections from WNT reporter, TOPGAL mice demonstrated inhibition in WNT activity at day 7 post-infarct with GNF-6231 treatment. Scale bars equal 50 μm. Images are representative of sections from N ≥ 3 mice; at least 4 areas were imaged from each mouse.