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. 2016 Dec 22;23(12):1550–1559. doi: 10.1016/j.chembiol.2016.11.008

Figure 1.

Figure 1

Design and Characterization of the FRET-Based Multi-Parameter Imaging Platform

(A) Method workflow.

(B) Representative FRET ratio traces in HeLa cells expressing sensors for monitoring Ras/ERK/RSK (left) and PDK1/Akt/S6K (right) pathways. Cells were stimulated with EGF (100 ng/mL) at time 0. Data represent mean ± SEM of four independent experiments. The number of cells analyzed is given in parentheses.

(C) Hierarchical clustering of the EGF (100 ng/mL) response amplitude over time. Clusters of responses are color-coded: strong (red), middle (blue), and weak (green). For each FRET biosensor the mean of four independent experiments is represented.