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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Toxicol In Vitro. 2015 Dec 10;32:123–131. doi: 10.1016/j.tiv.2015.12.002

Fig. 2. Detected levels of phthalate metabolites in testis co-culture after exposure to parent compounds.

Fig. 2

Testis co-cultures (and matrigel-only controls) were exposed to 100µM concentrations of either (a) dibutyl phthalate (DBP) or (b) bis(2-ethylhexyl) phthalate (DHEP) or (c) 150µM diethyl phthalate (DEP). After 2, 8 or 24 hours cell media, cell lysate and corresponding matrigel-only control samples were collected and analyzed for levels of monoester metabolites. Bars show means +/− s.e. (n=3 biological replicates for phthalate exposures and 2 replicate matrigel preparations). *indicates significant difference from corresponding matrigel control (p<0.05, t-test).