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. 2016 Dec 28;11(12):e0168572. doi: 10.1371/journal.pone.0168572

Fig 1. Hypoxia accentuates LOX expression via HIF-1, leading to enhanced collagen cross-linking.

Fig 1

(A) Hypoxic exposure increases expression of HIF-1α in the nuclear extract of wild-type mouse hepatocytes. This change is abolished in hepatocytes from Hif1a-/-hep mice. (B) LOX mRNA expression increases in wild-type hepatocytes exposed to hypoxia, but this increase in LOX is not observed in Hif1a-/-hep hepatocytes. (C) When culture media from wild-type hepatocytes is added to collagen, hypoxia causes increased collagen cross-linking and precipitation; this difference is not seen in Hif1a-/-hep hepatocytes. *, p<0.05; ††, p<0.001.