Figure 4.

Treatment with MSCs and GLP-1 modulates inflammation in NOD mice. Following treatment with MSCs and GLP-1 or MSCs alone, some mice from the groups of diabetic mice were euthanized at the indicated time points post treatment and the degree of inflammatory infiltrates in the pancreatic islets was examined. The frequency of splenic CD4⁺ and CD8⁺ T cells and CD4⁺CD25⁺Foxp3⁺ Tregs was determined by flow cytometry. Data are expressed as the mean ± SEM of individual group of mice (n = 6 per group per time point) from three separate experiments. (A) Insulitis levels. (B) Percentages of splenic CD4⁺ T cells. (C) Percentages of splenic CD8⁺ T cells. (D) Percentages of splenic Tregs. *P < 0.05 versus diabetic controls. †P < 0.05 versus mice treated with MSCs alone.