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. 2016 Dec 28;11(12):e0168739. doi: 10.1371/journal.pone.0168739

Fig 2. Effect of ENOblock on Enolase activity using spectrophotometric detection of PEP.

Fig 2

The effect on ENOblock (a) and SF2312 (c) on Enolase activity in purified recombinant ENO1 and ENO2 was determined using the direct PEP-detection assay. Panels a and c show enolase activity normalized to vehicle control and expressed as a function of inhibitor concentration. Each data point represents mean of N = 4 ± S.D. Panel b shows a representative traces of absorption at 240 nm over time of vehicle control; 62,000 nM ENOblock and 100 nM SF2312. Note the increased baseline with ENOblock. Panel d shows western blots for recombinant proteins blotted with ENO 1, ENO2 and pan-ENO antibodies.