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A SDS-PAGE gel of oxidized CtDsbA (3 μM) incubated for 24 h with increasing concentration of DTT (0 μM -12 mM). B The fraction of thiolate as a function of reduced DTT versus oxidized DTT is plotted. Fitting of the data revealed a Keq of 3.8 ± 0.8 x 10−4 M equivalent to a redox potential of -229 mV. Mean and SD calculated from 4 biological replicates are plotted.
