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. 2016 Dec 28;11(12):e0168485. doi: 10.1371/journal.pone.0168485

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© 2016 Christensen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — ecdsbA null E. coli are unable to swarm on soft agar. Exogenous expression of EcDsbA under an arabinose inducible promoter is able to complement this non-motile phenotype as shown by the swarming halo around the original inoculation point (upper panel, EcDsbA induced). CtDsbA is not able to restore the phenotype. Neither EcDsbA nor CtDsbA are able to restore mobility in a ecdsbA/ecdsbB double null E. coli strain (lower panels) indicating the requirement for EcDsbB to maintain a pool of oxidized DsbA.