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. 2016 Dec 28;11(12):e0168968. doi: 10.1371/journal.pone.0168968

Fig 3. Selection and characterization of HCT-116 Cas9 clones.

Fig 3

(A) Clones transfected with synthetic crRNA targeting GMNN exhibit increased nuclear area as compared to the parental HCT-116 Cas9 population. All exhibited a significant increase for crGMNN versus non-targeting control (p < 0.05) (B) Western blot indicates a greater decrease in GMNN protein for clones transfected with crGMNN versus parental HCT-116 Cas9 cells. Clones also exhibit greater levels of Cas9. (C) NGS sequencing of the GMNN target region in transfected cells (Clone-A) indicates >80% editing of GMNN DNA. (D) Effects of crRNAs targeting control genes known to affect nuclear area in HCT-116 Cas9 polyclonal and clonal populations. Bars represent the average and standard deviation of four replicates. The dashed line indicates five standard deviations above non-targeting control. Data for additional genes can be found in S2 Fig.