Figure 6.

DMA decreases transcriptional activity of NF-κB but not AP-1 or C/EBP. Luciferase activity was evaluated using the DualGlo luciferase reagent system after DMA and BAY 11-7082 in LPS-stimulated HEK 293/TLR4 cells transiently transfected with (A) NF-κB-Luc, (B) AP-1-Luc and (C) C/EBP-Luc reporter plasmids. The activity of Renilla-Luc was used to normalize the data. DMSO was included as a vehicle control. Data shown are means ± SEM of at least three independent experiments done in triplicate. ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.