Figure 2. Prdx3 may not interrupt formation of the TRAF6-ECSIT complex. (A) HEK293T cells were transiently transfected with mock, Flag-TRAF6, Myc-ECSIT, or Myc-Prdx3 as indicated. After 38 h, an immunoprecipitation (IP) assay with an anti-Myc antibody was performed, followed by immune blotting (IB) with anti-Myc or anti-Flag antibodies. The western blotting shown is representative of three independent experiments. (B) HEK293T cells were transfected with mock, Myc-Prdx3, Flag-TRAF6 wild type (wt), Flag-TRAF6 110-522, Flag-TRAF6 260-522, or Flag-TRAF6 349-522, as indicated. At 38 h after transfection, cells were extracted, immunoprecipitated with an anti-Flag antibody, and an immune blotting (IB) assay performed with anti-Flag or anti-Myc antibodies. The western blotting shown is representative of three independent experiments. (C) A schematic model for how TRAF6 interacts with Prdx3 and ECSIT.
