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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Stem Cells. 2016 Jul 27;35(1):158–169. doi: 10.1002/stem.2463

Figure 3. Neural differentiation and characterization of NPCs derived from reporter and parental lines.

Figure 3

(A) Schematic diagram of neural differentiation. (B) Differentiated NPCs are positive for neural progenitor markers, NESTIN and PAX6, as assessed by immunofluorescence (scale bar = 100 μm). (C) NPCs derived from the FXS reporter line were expanded for 23 passages and then differentiated into neurons (Tuj1+, red; scale bar = 50 μm). (D) Relative FMR1 mRNA levels assessed by quantitative RT-PCR. The mean expression of FX-iPSC-Nluc1 cells was set as 1 (Y axis in log scale; n=3; mean ± SEM; * indicates significant p value < 0.05). (E) A representative western blot result showing FMRP (arrow) and GAPDH (asterisk). (F) FMRP in reporter and parental NPCs detected using immunofluorescence and confocal imaging (scale bar = 100 μm).