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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Stem Cells. 2016 Jul 27;35(1):158–169. doi: 10.1002/stem.2463

Figure 6. Platform setup and library screening in 1536-well plates.

Figure 6

(A) Nluc activity from a 1536-well plate treated with DMSO in quadrant 2 and 4 and 5-aza-dC in quadrant 1 and 3. The Z’=0.57 for the screen. (B) Cells in a corner well (A1) and a center well (P24) in 1536-well plates were imaged in bright field on day 2 (the day after plating) and day 5 (the day of cell collection and luciferase assay), showing good cell survival and growth in 1536-well plates (scale bar = 400 μm). Larger images are shown in Figure S3. (C) Z’-factors were calculated for each column and each row of the 1536-well plate. (D) Screening of an FDA-approved Compound Library (1134 compounds, 3 μM) in 1536-well plates. Columns 23 and 47 were treated with negative control DMSO. Columns 24 and 48 were treated with positive control 5-aza-dC. Well A25 was 5-aza-C and well I11 was 5-aza-dC.