Fig. 2.

SIA and ANB-NOS crosslinking of GP and PhK. (A) Coomassie-stained 4-12% SDS-PAGE gel and Western blot analysis of SIA crosslinking products. GP and PhK were crosslinked individually to determine intramolecular crosslinking by SIA in lanes 1 and 3, respectively. In lane 2, PhK and GP were crosslinked together with SIA. The major GP-PhK conjugates formed by SIA crosslinking are labeled. The Western blot was carried out as described under Fig. 1 and Materials and Methods. (B) Same as (A), but with ANB-NOS crosslinking. (C) Side-by-side comparison of the co-migrating GP-α and GP-β conjugates formed with all three crosslinkers on Coomassie stained 4-12% SDS-PAGE gel.