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. Author manuscript; available in PMC: 2018 Jan 8.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Nov 11;482(2):221–225. doi: 10.1016/j.bbrc.2016.11.044

Fig. 3.

Fig. 3

Influence of PhK effectors on crosslinking by DFDNB. PhK and GP were crosslinked with DFDNB for 5 min in the presence of various effectors after pre-incubation of GP with DFDNB for 2 min. (A) Coomassie-stained GP-α and GP-β conjugates on 4-12% SDS-PAGE gel after crosslinking in the presence of the indicated small molecules. (B) Densities of GP-α (closed bars) and GP-β (open bars) after crosslinking in the absence or presence of effectors and normalized through dividing by the average density of the control GP-β conjugate. Error bars represent the standard deviation of triplicate samples. The lanes in part (A) came from the same experiment and gel. ND, none detected.