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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Arthritis Rheumatol. 2017 Jan;69(1):225–238. doi: 10.1002/art.39820

Figure 3.

Figure 3

Relationship between IL-10-producing and proinflammatory cytokine-producing B cells. B cells purified from a healthy 12-year-old child (HC17, Supplementary Table 1) were cultured with CpG for 5 hours (B10 cells) or CD40L+CpG for 48 hours (B10+B10PRO cells), with BFA or PIB added to the cultures during the last 5 hours. B cells were stained intracellularly for IL-10 and either GM-CSF (A), IL-1β (B) or IFN-γ (C). The position of all gates was determined using isotype-matched control mAb staining and fluorescence minus one (FMO) controls. These data are representative of those obtained in three separate experiments (n=3; HC3, HC7 and HC17 in Supplementary Table 1). All flow cytometry dot plots were gated on CD19+ live lymphocytes, with numbers representing their frequencies (% of total) within each gate; mean (±SEM; n=3) values are shown within parentheses in italics.