Table 1.
Bacterial strains and plasmids used in this study
| Strains and plasmids | Relevant features | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli strains | ||
| E. coli XL1‐Blue | (supE44 hsdR17 recA1 gyrA96 thi relA1 lac F′ [proAB + lacl q lacZΔM15 Tn10(Tetr)]) | Stratagene |
| E. coli XL1‐Blue‐pQE‐GadB | XL1‐Blue harboring pQE60 derivative containing gadB | This study |
| E. coli XL1‐Blue‐pQE‐GadB‐T | XL1‐Blue harboring pQE60 derivative containing gadB, which has mutation of Threonine225 to alanine | This study |
| E. coli XL1‐Blue‐pQE‐GadB‐D | XL1‐Blue harboring pQE60 derivative containing gadB, which has mutation of Aspartate256 to alanine | This study |
| E. coli XL1‐Blue‐pQE‐GadB‐K | XL1‐Blue harboring pQE60 derivative containing gadB, which has mutation of Lysine289 to alanine | This study |
| Bifidobacterium sp. strains | ||
| B. dentium ATCC27678 | ATCC | |
| B. angulatum ATCC27535 | ||
| B. bifidum MIMBb13CS | ||
| B. bifidum MIMBb23SG | ||
| B. breve NCIMB8807(also known as UCC2003) | Isolate from nursling stool | NCIMB |
| B. breve NCIMB8807+pESH‐GadB | Recombinant B. breve NCIMB8807 harboring pESH40 derivative containing gadB | This study |
| Lactobacillus sp. strains | ||
| L. acidophilus | ||
| L. farciminis DSM | DSMZ | |
| L. gasseri ATCC33323 | ATCC | |
| L. plantarum NCIMB8826 | NCIMB | |
| L. plantarum ATCC14917 | ATCC | |
| L. reuteri ATCC23272 | ATCC | |
| L. reuteri DSM17932 | DSMZ | |
| L. reuteri ATCC PTA 6475 | ATCC | |
| L. reuteri 100‐23 | Su et al.34 | |
| L. rhamnosus ATCC53103 (GG) | ATCC | |
| Alistipes sp. strains | ||
| A. indistinctus DSM22520 | DSMZ | |
| A. finegoldii DSM17242 | DSMZ | |
| A. putredinis DSM17216 | DSMZ | |
| Plasmids | ||
| pQE60 | Ampr, IPTG‐inducible expression vector | De Ruyter et al.78 Qiagen |
| pQE‐gadB | Ampr, pQE60 derivative containing translational fusion of gadB‐encoding DNA fragment to IPTG‐inducible promoter | This study |
| pQE‐gadB‐A | Ampr, pQE60 derivative containing translational fusion of gadB‐encoding DNA fragment to IPTG‐inducible promoter | This study |
| pQE‐gadB‐B | Ampr, pQE60 derivative containing translational fusion of gadB‐encoding DNA fragment to IPTG‐inducible promoter | This study |
| pQE‐gadB‐C | Ampr, pQE60 derivative containing translational fusion of gadB‐encoding DNA fragment to IPTG‐inducible promote | This study |
| pESH46 | Emr, Ampr, repA−, ori+, cloning vector | Shkoporov et al.47 |
| pESHGadB | Emr, Ampr, pESH46 derivative containing translational fusion of gadB‐encoding DNA fragment | This study |
ATCC, American Type Culture Collection; DSMZ, German Collection of Microorganisms and Cell Cultures; JCM, Japan Collection of Microorganisms; NCIMB, National Collection of Industrial and Marine Bacteria; MIM, collection from University of Milan.