Table 2.
Oligonucleotide primers used in this study
| Primer name | Sequence code (5′→3′) |
|---|---|
| GadBFw | AGCTCTCCATGGCGATAATGCATTCCAACATCAa |
| GadBRv | AGCTCTAAGCTTTCAGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGAAGCCGGAGACCCb |
| GadB‐T‐Fw | CATGGGCGTGGCCTACACGc |
| GadB‐D‐Fw | CCACGTGGCTGCCGCTTCGd |
| GadB‐K‐Fw | CGGACACGCGTACGGTCTGe |
| GadB‐T‐Rv | CGTGTAGGCCACGCCCATGf |
| GadB‐D‐Rv | CGAAGCGGCAGCCACGTGGg |
| GadB‐K‐Rv | CAGACCGTACGCGTGTCCGh |
| GadB‐XhoI‐Fw | CAACTCGAGGGAAACAGAAGGTTTGACAGi |
| GadB‐BamHI‐Fw | CTGGGATCCTCAGTGATGGAAGCCGj |
| Bd_gadB_pL_4_L | GCCAAGGCCTTCTCTAAGTTC |
| Bd_gadB_pL_4_R | CCACCTGATAGGCGGTCTC |
| bglC_pL_6_L | TGGAAGGACGAGGTGACTGk |
| bglC_pL_6_R | CTACGCGCTTCGGATCATk |
| h‐gabra1_pL_2_L | GGACAAACAGTAGACTCTGGAATTG |
| h‐gabra1_pL_2_R | TCAAGTGGAAATGAGTGGTCA |
| h‐gabrb2_pL_27_L | ATGGCACCGTCCTTTATGG |
| h‐gabrb2_pL_27_R | TGGGTACCTCCTTAGGTCCA |
| h‐gabbr1_pL_87_L | CATGGACGCTTATCGAGCA |
| h‐gabbr1_pL_87_R | GATCATCCTTGGTGCTGTCA |
| h‐gabbr2_pL_1_L | GACACCATCAGGTTCCAAGG |
| h‐gabbr2_pL_1_R | GATGCTGTAGAGAGGTAGGGAGA |
| h‐gabdh_pL_45_L | GAGTCCACTGGCGTCTTCAC |
| h‐gabdh_pL_45_R | TTCACACCCATGACGAACAT |
The underlined sequence corresponds to an NcoI site.
The underlined sequence corresponds to an HindIII site.
The underlined sequence corresponds to the introduced mutation site for Threonine.
The underlined sequence corresponds to the introduced mutation site for Aspartate.
The underlined sequence corresponds to the introduced mutation site for Lysine.
The underlined sequence corresponds to the introduced mutation site for Threonine.
The underlined sequence corresponds to the introduced mutation site for Aspartate.
The underlined sequence corresponds to the introduced mutation site for Lysine.
The underlined sequence corresponds to an XhoI site.
The underlined sequence corresponds to an BamHI site.
B. breve NCIMB8807 strain‐specific primers have been described previously by Pokusaeva et al.79