Skip to main content
. Author manuscript; available in PMC: 2017 May 21.
Published in final edited form as: Nat Genet. 2016 Nov 21;49(1):87–96. doi: 10.1038/ng.3728

Figure 3. Inactivation of CIC de-represses an ETV4-MMP24 pro-metastatic circuit.

Figure 3

(a) Inactivation of CIC de-represses ETV4 mediated transcription of ECM remodeling genes. (b) ChIP-PCR in H1975 and H1975 M1 cells demonstrating ETV4 promoter occupancy by CIC in lung cancer cells. p-values, Student’s t-test; error bars represent SEM. Data shown are representative of two independent experiments. (c) Progression-free survival for patients with LN positive (N1) NSCLC with high or low MMP24 mRNA expression. n=130; p=0.0016. Probe 213171_s_at. (d) MMP24 IHC score of matched primary lung tumors and LN metastases from two independent TMAs. n=32 pairs (32 primary tumors, 32 LN metastasis). Mean 1.03 +/− 0.14 (primary) and 2.25 +/− 0.16 (LN metastasis). p value calculated by Student’s t-test. (e) Immunoblots of H1975 parental cells expressing either shCtrl or shCICa, shCICb, or shCICc. (f) BLI of mice orthotopically implanted with H1975 cells expressing either Luciferase control (n=5), shCIC b (n=6), or shCIC c (n=7). (g) Metastasis-free surival of orthotopically implanted mice from (f). p values, log-rank. (h) Immunoblots of H1975 M1 cells expressing either GFP control or GFP-CIC. (i) Invasion of H1975 M1 cells expressing GFP control, GFP-CIC, or GFP-CIC with restored MMP24 expression (+MMP24). p-values, one-way ANOVA. Error bars represent SEM.