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. Author manuscript; available in PMC: 2017 May 21.
Published in final edited form as: Nat Genet. 2016 Nov 21;49(1):87–96. doi: 10.1038/ng.3728

Figure 4. CIC effector MMP24 drives lung cancer metastasis.

Figure 4

(a) BLI of shCtrl, shMMP24a, or shMMP24b expressing H1975 M1 mice. (b) Metastasis-free survival of H1975 M1 shCtrl (n=7), shMMP24a (n=12), or shMMP24b (n=7) mice. p-values, log-rank. (c) Circulating GFP+ cells/100 µl of blood at 5 weeks post-implantation. Mean +/− SEM, 51 +/− 6.1 (shCtrl, n=5), 20 +/− 4.5 (shMMP24a, n=7), 24 +/− 5.2 (shMMP24b, n=7). p-values, one-way ANOVA. (d) MMP24 staining of an orthotopically implanted tumor and mouse lung. T = tumor, L = lung. Scale bar, 50 µm (e) Tumor markers at the invasive edge. T = tumor, L = lung. Scale bar, 20 µm. (f) BLI of EV or MMP24 overexpressing H1975 GFP-Luc mice. (g) Metastasis-free survival of EV or MMP24-overexpressing H1975 mice. p-value, log rank. (h) Circulating GFP+ cells/100 µl of blood at 5 weeks post-implantation. Mean +/− SEM, 15 +/− 1.8 (EV, n=4) and 112 +/− 9.7 (+MMP24, n=6). p-value, Student’s t-test. (i) H&E of H1975 +MMP24: primary tumor (left lung), metastasis (right lung and hind limb). Scale bars, 100 µm (left) and 50 µm (right). (j) BLI of shCtrl or shMMP24a A549 GFP-Luc mice. (k) Metastasis-free survival of shCtrl (n=9) or shMMP24a (n=9) expressing A549 mice. p values, log-rank. (l) MRI of mice orthotopically implanted with a LA PDX. Circle = primary, arrows = metastasis. H (heart), R (right lung), L (left lung). (m) Invasion of EGFRL858R PDX +/− MMP24 knockdown. p-values, student’s t-test. Error bars represent SEM.