Table 3.
Matrix effect (ME) and extraction recovery at low, medium, and high concentrations in rat whole blood
| Compound | Low concentration | Medium concentration | High concentration | IS | ||||
|---|---|---|---|---|---|---|---|---|
| ME (%) | ER (%) | ME (%) | ER (%) | ME (%) | ER (%) | ME (%) | ER (%) | |
| Cocaine | 62.3 ± 8.3 | 78.8 ± 19.2 | 82.2 ± 3.8 | 97.1 ± 7.4 | 81.8 ± 3.2 | 77.8 ± 8.2 | 84.2 ± 4.9 | 73.9 ± 3.0 |
| EME | 23.2 ± 3.4 | 87 ± 8.6 | 31.2 ± 1.8 | 82.9 ± 5.6 | 34.6 ± 2.3 | 87.9 ± 3.4 | 23.6 ± 1.4 | 79.4 ± 5.6 |
| BE | 104.1 ± 7.4 | 96.2 ± 6.8 | 82.8 ± 4.5 | 112.5 ± 9.3 | 65.5 ± 2.8 | 87.9 ± 3.4 | 87.8 ± 4.8 | 81.4 ± 5.2 |
| ECG | 67.6 ± 6.5 | 95.1 ± 18.9 | 70.2 ± 4.7 | 113.9 ± 3.8 | 71.8 ± 3.7 | 90.8 ± 3.5 | 91.2 ± 5.6 | 84.2 ± 7.5 |
| Norcocaine | 82.1 ± 6.1 | 73.1 ± 3.1 | 88.3 ± 4.8 | 92.2 ± 8.3 | 83.8 ± 4.4 | 83.9 ± 8.9 | 84.2 ± 4.9 | 73.9 ± 3.0 |
| NEME | 52.1 ± 5.2 | 66.7 ± 11.3 | 57.7 ± 5.2 | 98 ± 6.9 | 59 ± 4.3 | 91.5 ± 5.0 | 84.2 ± 4.9 | 73.9 ± 3.0 |
| Cocaethylene | 58.5 ± 4.8 | 73.9 ± 4.9 | 72.3 ± 2.6 | 90.3 ± 2.9 | 75.3 ± 4.1 | 76.8 ± 6.3 | 59.7 ± 4.8 | 72.9 ± 4.3 |
| EEE | 75.6 ± 7.7 | 81.3 ± 10.2 | 82.6 ± 6.7 | 103.7 ± 8.2 | 84.7 ± 4.2 | 86.4 ± 4.5 | 84.2 ± 4.9 | 73.9 ± 3.0 |
| Norcocaethylene | 79.9 ± 10.6 | 69.1 ± 4.7 | 77.7 ± 5.7 | 92.8 ± 2.8 | 75.9 ± 4.3 | 76.8 ± 8.4 | 84.2 ± 4.9 | 73.9 ± 3.0 |
| NEEE | 84.3 ± 13.2 | 86.5 ± 11.5 | 81.6 ± 10.1 | 100.3 ± 13.8 | 81.8 ± 10.9 | 96.7 ± 9.1 | 84.2 ± 4.9 | 73.9 ± 3.0 |
Low concentration: 1.9–3.2 ng/ml (0.01 µM); Medium concentration: 95–160 ng/ml (0.5 µM); High concentration: 190–320 ng/ml (1 µM)