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. 2016 Dec 23;7:13755. doi: 10.1038/ncomms13755

Figure 1. PHLDB3 is induced by chemotherapy drugs in a p53-dependent manner.

Figure 1

(ad) Analysis of PHLDB3 mRNA and protein levels following chemotherapy drugs treatment. HCT116, H1299 and H460 cells were treated with Doxorubicin (Dox) or 5-Fluorouracil (5-Fu) for 16 h for analyses of RNA and protein levels. The mRNA levels of PHLDB3 were measured using RT-qPCR (a). The protein levels of PHLDB3, p53 and p53 targets were detected using immunoblotting analysis with indicated antibodies (bd). (e,f) Analysis of PHLDB3 mRNA and protein levels after p53 knockdown. H460 cells were transfected with p53 or scramble siRNA, and cells were harvested 72 h post-transfection for RT-qPCR (e) or immunoblotting with indicated antibodies (f). (g,h) The effect of p53 knockdown on the PHLDB3 mRNA (g) and protein levels (h) after treatment of HCT116p53+/+ cells with chemotherapy drugs. HCT116p53+/+ cells were transfected with p53 or scramble siRNA for 72 h, and treated with Dox or 5-Fu for 16 h before the cells were harvested for RT-qPCR (g) or immunoblotting with indicated antibodies (h). Where applicable, data were corrected for GAPDH and represent mean±s.e.m. of triplicate experiments. *P<0.05 by two-tailed t-test (a,e,g).