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. 2016 Dec 23;7:13755. doi: 10.1038/ncomms13755

Figure 7. PHLDB3 reduces the sensitivity of colorectal cancer cells to chemotherapeutic drugs.

Figure 7

(a,b) the effect of PHLDB3 overexpression on apoptosis in HCT116p53+/+ following drugs treatment. HCT116p53+/+ cells were transfected with PHLDB3-Flag or vector plasmid and then treated with Dox or 5-Fu for 16 h before harvesting. Cells were harvested 48 h post-transfection for flow cytometry analysis (a) or immunoblotting with indicated antibodies (b). Quantification of Sub-G1 population is shown in a. Data represent mean±s.e.m. of triplicate experiments.*P<0.01 by two-tailed t-test. The relative quantification of p53 protein level is shown in b. (c–f) Knockdown of PHLDB3 causes more drastic suppression of cell viability in p53-containing cancer cells than in p53-null cancer cells. HCT116p53+/+ (c,d) or HCT116 p53−/− (e,f) cells were transfected with PHLDB3 or control siRNA, and seeded in 96-well plates next day. Dox or 5-Fu was supplemented for 72 h before cell viability detection by CCK-8.